Montag, 28. Juni 2010

Installing bioperl without root access

In order to give our users the maximum freedom, we are not installing perl modules systemwide, but the modules have to be installed on a per user basis, unless the modules are of such importance and wide use by our user basis, that it justifies a system wide installation. Here is a tutorial how to install perl modules by yourself without having root access:

1) start cpan and config. defaults are ok.
downloadsites set: 11 9 4
takes a while -> quit

2) create local perl module directory:
> mkdir ~/myperl

3) start cpan and set the following options:

> cpan
cpan> o conf makepl_arg "LIB=~/myperl/lib \
INSTALLMAN1DIR=~/myperl/man/man1 \
INSTALLMAN3DIR=~/myperl/man/man3 \
INSTALLSCRIPT=~/myperl/bin \

cpan> o conf mbuildpl_arg "--lib=~/myperl/lib \
--installman1dir=~/myperl/man/man1 \
--installman3dir=~/myperl/man/man3 \
--installscript=~/myperl/bin \

cpan> o conf mbuild_install_arg "--install_path lib=~/myperl"
cpan> o conf prerequisites_policy automatically
cpan> o conf commit
cpan> quit

then you can install whatever modules you like in your local directory.
For example, for bioperl you would need:

cpan>d /bioperl/
CPAN: Storable loaded ok
Going to read /home/bosborne/.cpan/Metadata
Database was generated on Mon, 20 Nov 2006 05:24:36 GMT


Distribution B/BI/BIRNEY/bioperl-1.2.tar.gz
Distribution B/BI/BIRNEY/bioperl-1.4.tar.gz
Distribution C/CJ/CJFIELDS/BioPerl-1.6.0.tar.gz

Now install:

cpan> force install C/CJ/CJFIELDS/BioPerl-1.6.0.tar.gz

in case the download is slow, then edit the file ~/.cpan/CPAN/ and insert the following line
into $CPAN::Config :

'dontload_hash' => { "Net::FTP" => 1, "LWP" =>1 },

in case something goes wrong you can delete ~/.cpan and start over again.

very helpful is the perl shell which you can obtain by installing:
cpan> install Psh
cpan> install IO::String

it is then available under ~/myperl/bin/psh
e.g. try out
psh% use Bio::Perl;
psh% $seq_object = get_sequence('genbank',"ROA1_HUMAN");
psh% write_sequence(">roa1.fasta",'fasta',$seq_object);

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